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draft contig sequences of pvef4  (Macrogen)

 
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    Macrogen draft contig sequences of pvef4
    Genetic map of pIP816 and <t>pVEF4.</t> Coding regions are represented by arrows indicating the direction of transcription and are coloured according to their predicted functions. The inverted repeats (IR) of the Tn 1546 transposon and the predicted origin of replication ( oriR ) of the plasmids are given as black boxes. The group II intron En . fm .I2 of pVEF4 is shown as dark grey boxes flanking the intron-encoding protein. Thin arrows indicate the 25 kb larger genetic unit. Truncated CDSs are indicated with a prime symbol (e.g. tnp ′).
    Draft Contig Sequences Of Pvef4, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/draft+contig+sequences+of+pvef4/draft+contig+sequences+of+pvef4/pmc02920175-170-5-9
    Average 90 stars, based on 1 article reviews
    draft contig sequences of pvef4 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Tn 1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages"

    Article Title: Tn 1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages

    Journal: Journal of Antimicrobial Chemotherapy

    doi: 10.1093/jac/dkq219

    Genetic map of pIP816 and pVEF4. Coding regions are represented by arrows indicating the direction of transcription and are coloured according to their predicted functions. The inverted repeats (IR) of the Tn 1546 transposon and the predicted origin of replication ( oriR ) of the plasmids are given as black boxes. The group II intron En . fm .I2 of pVEF4 is shown as dark grey boxes flanking the intron-encoding protein. Thin arrows indicate the 25 kb larger genetic unit. Truncated CDSs are indicated with a prime symbol (e.g. tnp ′).
    Figure Legend Snippet: Genetic map of pIP816 and pVEF4. Coding regions are represented by arrows indicating the direction of transcription and are coloured according to their predicted functions. The inverted repeats (IR) of the Tn 1546 transposon and the predicted origin of replication ( oriR ) of the plasmids are given as black boxes. The group II intron En . fm .I2 of pVEF4 is shown as dark grey boxes flanking the intron-encoding protein. Thin arrows indicate the 25 kb larger genetic unit. Truncated CDSs are indicated with a prime symbol (e.g. tnp ′).

    Techniques Used:

    Coding sequences (CDSs) of the vanA plasmid pVEF4 (partial)
    Figure Legend Snippet: Coding sequences (CDSs) of the vanA plasmid pVEF4 (partial)

    Techniques Used: Plasmid Preparation, Reverse Transcription, Infection

    Structural features of En . fm .I2 and its intron-encoded protein (IEP). (a) Predicted secondary RNA structure of En . fm .I2. Intron nucleotides are written in capital letters and exon sequences are written in lowercase letters. Roman numerals denote the domains I–VI. The IEP is found in domain IV. Intron-binding sites (IBSs) 1 and 2 along with the exon-binding sites (EBSs) 1 and 2 are marked by arrows and boxes, respectively. IBS/EBS3 is a single nucleotide interaction and denoted by pointing arrows. The bulged A (branch site) is located in domain VI and shown in bold. (b) The putative IEP displays a reverse transcriptase (RT) domain (bold letters), a maturase (X) domain (italics) and an endonuclease (En) domain (grey). All introns analysed, except two, had identical amino acid composition to En . fm .I2 of pVEF4 (no. 54, top). The non-synonymous substitutions in En . fm .I2 from E. faecium strains 31/F01/H (no. 49) and TUH32-79 (no. 45) are shown in the alignment (identical amino acids are represented by a dot; a dash indicates gaps or substitutions).
    Figure Legend Snippet: Structural features of En . fm .I2 and its intron-encoded protein (IEP). (a) Predicted secondary RNA structure of En . fm .I2. Intron nucleotides are written in capital letters and exon sequences are written in lowercase letters. Roman numerals denote the domains I–VI. The IEP is found in domain IV. Intron-binding sites (IBSs) 1 and 2 along with the exon-binding sites (EBSs) 1 and 2 are marked by arrows and boxes, respectively. IBS/EBS3 is a single nucleotide interaction and denoted by pointing arrows. The bulged A (branch site) is located in domain VI and shown in bold. (b) The putative IEP displays a reverse transcriptase (RT) domain (bold letters), a maturase (X) domain (italics) and an endonuclease (En) domain (grey). All introns analysed, except two, had identical amino acid composition to En . fm .I2 of pVEF4 (no. 54, top). The non-synonymous substitutions in En . fm .I2 from E. faecium strains 31/F01/H (no. 49) and TUH32-79 (no. 45) are shown in the alignment (identical amino acids are represented by a dot; a dash indicates gaps or substitutions).

    Techniques Used: Binding Assay, Reverse Transcription

    RT–PCR analyses of the topoisomerase, intron, intron splicing products and enterococcal elongation factor. RT–PCR products from pVEF4 and pVEF3 are given on alternate lanes 1–10. PCR products are shown as follows: topo mRNA without intron (lanes 1 and 2, primer pair ip3F/giiR7); 5′ intron–exon junction (lanes 3 and 4, primer pair ip3F/giiR8); 3′ intron–exon junction (lanes 5 and 6, primer pair giiF5/giiR7); intron lariat structure (lanes 7 and 8, primer pair giiF5/giiR8); and positive RT–PCR control (lanes 9 and 10, primer pair Ent1/Ent2). Ladder (lanes L), 100 bp DNA molecular size marker from New England Biolabs. The sequence data of the ligated exon with the indicated splice site is shown in the lower half of the figure.
    Figure Legend Snippet: RT–PCR analyses of the topoisomerase, intron, intron splicing products and enterococcal elongation factor. RT–PCR products from pVEF4 and pVEF3 are given on alternate lanes 1–10. PCR products are shown as follows: topo mRNA without intron (lanes 1 and 2, primer pair ip3F/giiR7); 5′ intron–exon junction (lanes 3 and 4, primer pair ip3F/giiR8); 3′ intron–exon junction (lanes 5 and 6, primer pair giiF5/giiR7); intron lariat structure (lanes 7 and 8, primer pair giiF5/giiR8); and positive RT–PCR control (lanes 9 and 10, primer pair Ent1/Ent2). Ladder (lanes L), 100 bp DNA molecular size marker from New England Biolabs. The sequence data of the ligated exon with the indicated splice site is shown in the lower half of the figure.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Control, Marker, Sequencing

    Related Articles

    Sequencing:

    Article Title: Tn 1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages
    Article Snippet: .. The draft contig sequences of pVEF4 were provided by Macrogen, Korea, using BigDye chemistry and with a sequence depth of >14× coverage. ..



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    draft contig sequences of pvef4  (Macrogen)
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    Macrogen draft contig sequences of pvef4
    Genetic map of pIP816 and <t>pVEF4.</t> Coding regions are represented by arrows indicating the direction of transcription and are coloured according to their predicted functions. The inverted repeats (IR) of the Tn 1546 transposon and the predicted origin of replication ( oriR ) of the plasmids are given as black boxes. The group II intron En . fm .I2 of pVEF4 is shown as dark grey boxes flanking the intron-encoding protein. Thin arrows indicate the 25 kb larger genetic unit. Truncated CDSs are indicated with a prime symbol (e.g. tnp ′).
    Draft Contig Sequences Of Pvef4, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/draft+contig+sequences+of+pvef4/draft+contig+sequences+of+pvef4/pmc02920175-170-5-9
    Average 90 stars, based on 1 article reviews
    draft contig sequences of pvef4 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Genetic map of pIP816 and pVEF4. Coding regions are represented by arrows indicating the direction of transcription and are coloured according to their predicted functions. The inverted repeats (IR) of the Tn 1546 transposon and the predicted origin of replication ( oriR ) of the plasmids are given as black boxes. The group II intron En . fm .I2 of pVEF4 is shown as dark grey boxes flanking the intron-encoding protein. Thin arrows indicate the 25 kb larger genetic unit. Truncated CDSs are indicated with a prime symbol (e.g. tnp ′).

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Tn 1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages

    doi: 10.1093/jac/dkq219

    Figure Lengend Snippet: Genetic map of pIP816 and pVEF4. Coding regions are represented by arrows indicating the direction of transcription and are coloured according to their predicted functions. The inverted repeats (IR) of the Tn 1546 transposon and the predicted origin of replication ( oriR ) of the plasmids are given as black boxes. The group II intron En . fm .I2 of pVEF4 is shown as dark grey boxes flanking the intron-encoding protein. Thin arrows indicate the 25 kb larger genetic unit. Truncated CDSs are indicated with a prime symbol (e.g. tnp ′).

    Article Snippet: The draft contig sequences of pVEF4 were provided by Macrogen, Korea, using BigDye chemistry and with a sequence depth of >14× coverage.

    Techniques:

    Coding sequences (CDSs) of the vanA plasmid pVEF4 (partial)

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Tn 1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages

    doi: 10.1093/jac/dkq219

    Figure Lengend Snippet: Coding sequences (CDSs) of the vanA plasmid pVEF4 (partial)

    Article Snippet: The draft contig sequences of pVEF4 were provided by Macrogen, Korea, using BigDye chemistry and with a sequence depth of >14× coverage.

    Techniques: Plasmid Preparation, Reverse Transcription, Infection

    Structural features of En . fm .I2 and its intron-encoded protein (IEP). (a) Predicted secondary RNA structure of En . fm .I2. Intron nucleotides are written in capital letters and exon sequences are written in lowercase letters. Roman numerals denote the domains I–VI. The IEP is found in domain IV. Intron-binding sites (IBSs) 1 and 2 along with the exon-binding sites (EBSs) 1 and 2 are marked by arrows and boxes, respectively. IBS/EBS3 is a single nucleotide interaction and denoted by pointing arrows. The bulged A (branch site) is located in domain VI and shown in bold. (b) The putative IEP displays a reverse transcriptase (RT) domain (bold letters), a maturase (X) domain (italics) and an endonuclease (En) domain (grey). All introns analysed, except two, had identical amino acid composition to En . fm .I2 of pVEF4 (no. 54, top). The non-synonymous substitutions in En . fm .I2 from E. faecium strains 31/F01/H (no. 49) and TUH32-79 (no. 45) are shown in the alignment (identical amino acids are represented by a dot; a dash indicates gaps or substitutions).

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Tn 1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages

    doi: 10.1093/jac/dkq219

    Figure Lengend Snippet: Structural features of En . fm .I2 and its intron-encoded protein (IEP). (a) Predicted secondary RNA structure of En . fm .I2. Intron nucleotides are written in capital letters and exon sequences are written in lowercase letters. Roman numerals denote the domains I–VI. The IEP is found in domain IV. Intron-binding sites (IBSs) 1 and 2 along with the exon-binding sites (EBSs) 1 and 2 are marked by arrows and boxes, respectively. IBS/EBS3 is a single nucleotide interaction and denoted by pointing arrows. The bulged A (branch site) is located in domain VI and shown in bold. (b) The putative IEP displays a reverse transcriptase (RT) domain (bold letters), a maturase (X) domain (italics) and an endonuclease (En) domain (grey). All introns analysed, except two, had identical amino acid composition to En . fm .I2 of pVEF4 (no. 54, top). The non-synonymous substitutions in En . fm .I2 from E. faecium strains 31/F01/H (no. 49) and TUH32-79 (no. 45) are shown in the alignment (identical amino acids are represented by a dot; a dash indicates gaps or substitutions).

    Article Snippet: The draft contig sequences of pVEF4 were provided by Macrogen, Korea, using BigDye chemistry and with a sequence depth of >14× coverage.

    Techniques: Binding Assay, Reverse Transcription

    RT–PCR analyses of the topoisomerase, intron, intron splicing products and enterococcal elongation factor. RT–PCR products from pVEF4 and pVEF3 are given on alternate lanes 1–10. PCR products are shown as follows: topo mRNA without intron (lanes 1 and 2, primer pair ip3F/giiR7); 5′ intron–exon junction (lanes 3 and 4, primer pair ip3F/giiR8); 3′ intron–exon junction (lanes 5 and 6, primer pair giiF5/giiR7); intron lariat structure (lanes 7 and 8, primer pair giiF5/giiR8); and positive RT–PCR control (lanes 9 and 10, primer pair Ent1/Ent2). Ladder (lanes L), 100 bp DNA molecular size marker from New England Biolabs. The sequence data of the ligated exon with the indicated splice site is shown in the lower half of the figure.

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Tn 1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages

    doi: 10.1093/jac/dkq219

    Figure Lengend Snippet: RT–PCR analyses of the topoisomerase, intron, intron splicing products and enterococcal elongation factor. RT–PCR products from pVEF4 and pVEF3 are given on alternate lanes 1–10. PCR products are shown as follows: topo mRNA without intron (lanes 1 and 2, primer pair ip3F/giiR7); 5′ intron–exon junction (lanes 3 and 4, primer pair ip3F/giiR8); 3′ intron–exon junction (lanes 5 and 6, primer pair giiF5/giiR7); intron lariat structure (lanes 7 and 8, primer pair giiF5/giiR8); and positive RT–PCR control (lanes 9 and 10, primer pair Ent1/Ent2). Ladder (lanes L), 100 bp DNA molecular size marker from New England Biolabs. The sequence data of the ligated exon with the indicated splice site is shown in the lower half of the figure.

    Article Snippet: The draft contig sequences of pVEF4 were provided by Macrogen, Korea, using BigDye chemistry and with a sequence depth of >14× coverage.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Marker, Sequencing